NGS(Next Generation Sequencing)
1. NGS Systems [MGI]
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| Product Model | DNBSEQ-T20x2 | DNBSEQ-T7 | DNBSEQ-G400 | DNBSEQ-G400* For HotMps Only |
DNBSEQ-G50 | DNBSEQ-G99 |
| Dimensions | 4200mm*4800mm *2000mm |
1656mm*903mm *1815mm |
1086mm*756mm *710mm |
1086mm*756mm *710mm |
654mm*489mm* 654mm*489mm* |
654mm*489mm* *640mm |
| Applications | Ultra-high=depth Whole Geome Sequencing |
Deep Whole Genome Sequencing |
WGS, WES, Trascriptiome sequencing, etc. |
WGS, WES, Transcriptome sequencing, etc. |
Small whole Genome sequencing, targeted panels, low-pass whole genome sequencing |
Targeted oncology panel sequencing, infectious disease sequecing, 16S metagenomic Sequencing |
| Max. Flow Cell/Run | 6 | 4 | 2 | 2 | 1 | 2 |
| Flow Cell Type | Slide | FC | FCS&FCL | FCL | FCS&FCL | FC |
| Lane/Flow Cell++ | 1 Lane | 1 Lane | 4 Lanes or 2 Lanes | 4 Lane | 1 Lane | 1 Lane |
| Operation Mode | Ultra-high throughput | High and Ultra-high Throunghput |
Medium and High Throungput |
Medium and High Throughput |
Medium Throughput | Low and Medium Low and Medium |
| Max.Throunghput /Run |
~72Tb | 7Tb | 1400Gb | 720Gb | 150Gb | 96GB |
| Effective Reads/ Flow Cell |
35B(PE 100) | 5800M | 300M, 550M, 1500M~1800M |
1500M~1800M | 100M, 500M | 80M |
| Average Run Time |
60hrs~80hrs | 16hrs~24hrs | FCS : 13hrs~37hrs FCL : 14hrs~109hrs |
15.5hrs~50.5hrs | 9hrs~40hrs | 5hrs~30hrs |
| Min.Read Length |
PE100 | PE100 | SE50 | SE50 | SE50 | SE100 |
| Max. Read Length |
PE150 | PE150 | PE300 | PE100 | PE150 | PE300 |
2. NGS Library Automations
1) Revvity
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| System | 96 pipetting head, 12 deck positions [+27(3stackers)] |
96 pipetting head, 40 deck positions (24 for tip boxes, 16WS) |
96 pipetting head, 48 deck positions (21WS, 27 Tip Box(3 stackers), PCR sys. |
| Sample Capacity | 8 to 48/96 samples per run | 3 to 5 batches each with 8 to 96 samples per week(depending on application) |
3 to 5 batches each with 8 to 96 samples per week(192 samples depending on application) |
| Key Applications |
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| Ideally for... |
HT sample preparation (e.g. For NextSeq2000) [Full Automation System (Included PCR, Tip_Stacker(3X9), Twister ARM)] |
HT sample preparation (e.g. For NextSeq2000, NovaSeq6000) |
HT sample preparation (e.g. For NextSeq2000, NovaSeq6000) Full Automation System (Included PCR, Tip_Stacker(3X9), Twister ARM) |
2)MGI
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| Size | 780mmX725mmX777mm | 1240mmX740mmX1110mm | 1410mmX799mmX970mm |
| Weight | 130kg | 280kg | 220kg |
| Pipette Type | Fixed 8 channel | Fixed 96 channel | Independent 8-channel pipette |
| Throughput | Sandard : 16 samples/run | Sandard : 96 samples/run | Sandard : 8~48/96 |
| Pipette Range | 2㎕~200㎕ | 1㎕~200㎕ | 1㎕~1000㎕ |
| Pipette Accuracy | 2㎕<±10% 200㎕<±1% |
2㎕<±10% 200㎕<±1% |
1㎕<±10%; 200㎕<±2%; 1000㎕<±1% |
| Pipette CV | 2㎕<5% 200㎕:<1% |
2㎕<5% 200㎕:<1% |
1㎕:CV<8%; 200㎕:CV<1%; 10001㎕:CV<1% |
